Micropropagation of Tomato Hybrid cv. 'Falcato'

Abstract

This study was carried out to establish a micro propagation protocol for Falcato tomato hybrid in which several factors were studied including explant type, type and concentration of growth regulator on shoot multiplication, and rooting of explant on MS (Murashige and Skoog). The obtained results indicated that for multiplication of shoot tip explants, best results were achieved by using BA at 8.8 Micromole/liter with a mean of 1.9 shoot per explant. Shoot tip explants were also had a higher shoot length (3.2 cm) compared to single node explants. Control explants had 95% rooting, while using the auxins NAA and IBA gave 90% rooting. The results showed that there were significant differences between shoot tip and single node explants based on number of roots per explant, whereas shoot tip and single node explants had 11.6 and 11.0 root per explant respectively. Plantlets were successfully acclimatized.