Lactobacillus Species as a Potential Biomarker for Colorectal Cancer: Biopsy-Based Insights

Abstract

Colorectal cancer (CRC) is the third most prevalence type of cancer with the second mortality rate. CRC is a heterogenous
disease and several factors were correlated to its development such as diet, genetics, lifestyle and recently, accumulating
evidence advocate that the gut microbiota dysbiosis was an innovative and imperative player in the initiation and development
of colorectal cancer. On the other hands, many recent scientific research suggested the protective role of lactobacillus species
through different mechanisms. Such protective mechanisms ether by enhancing the immune response, suppressing the effect
of free radicals, control cell cycle and proliferations and etc. To study the correlation between Lactobacillus and CRC among
Libyan patients and wither such bactria play any role in the devolopment and progression in such cancer type This study was
conducted in the city of Tripoli, Libya. The study included, 20 CRC patients with colorectal cancer confirmed by colonoscopy
examination and evaluated histopathologically, 20 patients with large adenomas polyp and 20 people with healthy colonic mucosa
as control group. The sample of each patient which obtained by proximal to the colonoscopic part of the suction tube was sent to
the clinical microbiology. Laboratory for isolation according to the standard laboratory procedures guideline (CSLI, 2012). The
suspected positive colonies were isolated and identified by Gram staining technique and catalase test, and further confirmed by
the use of phoenix BD automated system. The results of this study revealing, significant declined of Lactobacillus salivarius
in polypic group (%5) and CRC groups (%5) while Lactobacillus salivarius of the normal mucosal group represent ( .(%35The
Lactobacillus rhamnosus also shown the same pattern of L. salivarius. The obtained result strongly affirming the protective role
of Lactobacillus species among Libyan CRC paients